Global Journal of Medical Research, F: Diseases, Volume 22 Issue 4

protein structure when available. Moreover, it can predict deleterious Single Nucleotide Polymorphism starting from the protein sequence alone. (Capriotti et al., 2006). ii. MUpro: http://mupro.proteomics.ics.uci.edu/ It is a machine-learning approach based on support-vector machines to predict the protein stability changes for single site mutations in two contexts taking into account structure-dependent and sequence- dependent information, respectively (Cheng et al., 2006). e) Prediction of protein modeling This was achieved by using project Hope sof twarehttps://www3.cmbi.umcn.nl/hope/. HOPE is a next-generation software application for automatic mutant analysis. HOPE was designed to explain the molecular origin of a disease-related phenotype caused by mutations in human proteins. HOPE collects information from data sources such as the protein’s 3D structure and the UniProt database of well-annotated protein sequences. For each protein, this data is stored in a PostgreSQL-based information system. A decision scheme is used to process these data and predict the effects of the mutation on the 3D structure and the protein's function (Das et al., 2022). III. R esults Using Gene MANIA, the HGD gene was found to have an association with 20 other different genes. Among these is the HPD gene which provides instructions for making the 4-hydroxyphenylpyruvate dioxygenase enzyme. This gene is the second in a series of five enzymes that work to break down the amino acid tyrosine, a protein-building block found in many foods. Figure (1) and Table (1). The physical interaction and co-expression of this gene with other related genes is shown in Figure (1). Figure 1: Gene MANIA result for HDG Gene Table 1: Gene Description Rank Using GeneMANIA Gene Description HGD homogentisate 1,2-dioxygenase HPD 4-hydroxyphenylpyruvate dioxygenase GSTZ1 glutathione S-transferase zeta 1 ALDH1L1 aldehyde dehydrogenase 1 family member L1 HSD17B10 hydroxysteroid 17-beta dehydrogenase 10 ADI1 acireductone dioxygenase 1 KMO kynurenine 3-monooxygenase CDO1 cysteine dioxygenase type 1 ADO 2-aminoethanethiol dioxygenase MPI mannose phosphate isomerase GGCX gamma-glutamyl carboxylase PIR Pirin CENPC centromere protein C HAAO 3-hydroxyanthranilate 3,4-dioxygenase G6PC glucose-6-phosphatase catalytic subunit 17 Year 2022 Global Journal of Medical Research Volume XXII Issue IV Version I ( D ) F © 2022 Global Journals Computational Analysis of Possibly Pathogenic Non-Synonymous Single Nucleotide Polymorphisms Variants in HGD Gene