Global Journal of Medical Research, G: Veterinary Science and Veterinary Medicine, Volume 21 Issue 2

Microbiological Evaluation of Poultry Meat Obtained from Different Retail Markets in Khulna District Bidyut Matubber α , Joyanta Kumar Das σ , Md. Ahsan Habib ρ , Sabuj Kanti Nath Ѡ & Md. Uzzal Hossain ¥ Abstract- The research work conducted to evaluate microbial load in poultry meat from different retail markets in the Khulna district (Nirala, Dumuria, and Fultola). The objective of the present study was to get quality poultry products from commercial farms and retail markets. A total of 48 samples collected and brought to Quality Feed Lab. for laboratory assay. Samples were propagating in nutrient agar followed by culture on selective media, Eosin Methylene Blue Agar, Mac- Conkey agar, Brilliant Green Agar, Salmonella-Shigella Agar. Total numbers of 48 samples examined, and 30 samples were found positive to E. coli , and the prevalence of E. coli in this study was 62.20 %. 37.50% of thigh meat samples were positive for E. coli within 24 tested samples whereas 87.50% of breast meat samples were positive within 24 tested samples for this bacteria. On the other hand, 23 samples were found positive for Salmonella spp, and the prevalence of Salmonella spp in this study was 49.91 %. 29.16% of thigh samples were positive for Salmonella spp within 24 tested samples whereas 66.66% of litter samples were positive within 24 tested samples for this bacteria. Total Viable Count (TVC), Total Coliform Count (TCC), Total Salmonella Count (TSC) ,and Total Campylobacter Count (CPC) in meat samples of different broiler markets like Nirala market at Khulna city corporation, Dumuria, and Fultola were determined. Mean of TVC, TCC, TSC, and TCpC for the Nirala market at Khulna city corporation, Dumuria and Fultola markets were 5.61, 5.84, 6.29 log10 CFU/g, 4.72, 4.50, 4.47 log10 CFU/g, 3.19, 3.44, 3.49, log10 CFU/g and 2.31, 2.50, 2.54 log10 CFU/g, respectively. It observed that the mean values of TVC, TCC, TSC and TCpC in the case of Dumuria, and Fultola market exceeded the ICMSF recommendations, which may cause alarm to consumer’s health. Author α : Lecturer, Department of Microbiology and Public Health, Khulna Agricultural University, Khulna, Bangladesh. e-mail: bidyutm78@gmail.com Author σ : MS, Department of Microbiology and Public Health, Patuakhali Science and Technology University, Patuakhali, Bangladesh. e-mail: joydvm99@gmail.com Author ρ ¥ : Lecturer, Department of Livestock Production and Management, Khulna Agricultural University, Khulna, Bangladesh. e-mail: ahhelal313@gmail.com Author Ѡ : Lecturer, Department of Animal Nutrition, Khulna Agricultural University, Khulna, Bangladesh. I. I ntroduction oultry meat is equally important as a microbiological safety and quality to producers, retailers, and consumers (Mead et al., 2004). Because of advantages such as easy digestibility and acceptance, poultry meat is becoming more popular in the consumer market by most people (Yashoda et al., 2001). However, chicken meat consists of high-quality protein and many other nutrients that are very important for body function (Kralik et al., 2017). Poultry meats to be optimally incorporated into the diet at all ages because of their high-biological-value protein, vitamin, and mineral content associated with a low-fat content (Marangoni et al., 2015). The consumption of poultry meat has increased worldwide as it’s a highly nutritious and safe food (Gonzalez-Ortiz et al., 2013). About 90 percent of the rural families are consuming small numbers of chickens (Das et al., 2008). Poultry meat contaminated by different types of microorganisms during processing in the processing plants (Maharjan et al., 2019). During slaughtering, poultry meat contaminated because of the malpractices in handling and management with remains foodborne pathogens remains important health-hazardous issue (Javadi and Safarmashaei et al., 2011). Chicken meat products from retail markets contaminated with foodborne pathogens, namely, Staphylococcus aureus , Salmonella, E. coli , and Listeria monocytogenes , and contamination with mold and yeasts (Khalafalla et al., 2019). Pathogenic strains of salmonella, S. aureus , S. epidermidis , shigella, enterobacter, and Citrobacter are serious health threats for a human beings (Alam et al., 2015). Foodborne pathogens are causing many diseases with significant effects on human health and the economy (Bintsis, 2017). The food-borne pathogen causes various of illness and death that loses billions of dollars for medical care, medical and social costs (Fratmico et al., 2005). Food-borne illnesses are still public health issue in both developing and developed countries despite applying many control and preventive measures (Zhou et al., 2010). The aim of this study to detect food-borne pathogens in Poultry meat of different areas in the Khulna district and to know the potential risk factor of food-borne pathogens in Khulna district. P Year 2021 Global Journal of Medical Research Volume XXI Issue II Version I ( DD ) G © 2021 Global Journals 7