Global Journal of Medical Research, L: Nutrition & Food Science, Volume 22 Issue 2

Antihyperlipidemic Property of a Dietary Supplement of Moringa Oleifera Leaves and Pleurotus Ostreatus in Wistar Rats Stressed by Combination of Ethanol-Paracetamol Fatou Corka Kane α , Simo Nemg Fredy Brice σ , Moundipa F. Paul ρ &Wilfred F. Mbacham Ѡ Abstract- High amounts of triglycerides and cholesterol in the blood result in the metabolic condition known as hyperlipidemia. There is currently no specific therapy to reduce the effects of this disorder. In underdeveloped nations, metabolic diseases are treated using Moringa oleifera and Pleurotus ostreatus . Both the nutritional and therapeutic benefits of these two plants are frequently utilized. Purpose: This study aimsto investigate the antihyperlipidemic property of dietary supplement of Moringa oleifera leaves and Pleurotus ostreatus in wistar rats. Materials and methods: A variety of mushroom species were produced in the Mushroom Biotechnology Laboratory, and M. oleifera was developed in the university's botanical garden in Dakar, Senegal. In this study, the extract of these two plants, designated FMP16, was used to treat rats that had been exposed to oxidative stress caused by the combination of ethanol and paracetamol as follows: control (TG), stressed (TP), ethanol-paracetamol treated groups (D1P- D2P- D3P), which received three doses of the supplement at 500 mg/kg, 1000 mg/kg, and 1500 mg/kg, followed by ethanol in five sequential doses of 2 g. To measure: oxidative stress parameters, total plasma cholesterol, triglycerides, low-density lipoprotein (LDL Cholesterol), and high-density lipoprotein (HDL Cholesterol), blood and liver samples were collected. Results: According to findings, giving rats a meal consisting of a 2:1 ratio of Moringa oleifera and Pleurotus ostreatus lowered plasma levels of total cholesterol, triglycerides, and low- density lipoprotein (LDL). Compared to TG, it decreased the LDL cholesterol of D1P, D2P, and D3P by 39%, 30%, and 38%, respectively. D2P's SGPT and SGOT concentrations were also decreased by 29% and 28%, respectively, compared to TP. The dosage of 1000 mg/kg would be the most suitable for liver damage. Conclusion: According to the results of the current study, taking M. oleifera leaves and P. ostreatus supplements may have health benefits, at least because they affect the lipid profile and liver damage in stressed rats. Author α σ ρ Ѡ : Department of Biochemistry, Faculty of Science, University of Yaoundé I, Cameroon. e-mail: fatoucorkakane@gmail.com Author α : Mushroom Biotechnology Laboratory, Department of Plant Biology and Physiology, Faculty of Sciences, Cheikh Anta Diop University of Dakar, Senegal. Author α Ѡ : Laboratory for Food and Drug Safety, and Public Health Biotechnologies, University of Yaoundé I, Cameroon. Author Ѡ : Centre for Health Implementation and Translational Research, The Fobang Institutes, Yaoundé, Cameroon. Keywords: moringa oleifera, pleurotus ostreatus, dietary supplement, antihyperlipidemic, oxidative stres s . I. I ntroduction lcoholism and other serious health issues are brought on by excessive alcohol usage, including alcoholic liver damage (ALD). Alcoholism has been linked to several illnesses, and it is currently one of the most challenging health issues with substantial medical, social, and economic repercussions. (Pari and Karthikesan, 2001; Sivaraj et al ., 2010). Alcohol abuse leads to significant illnesses such as hyperglycemia, cirrhosis, cardiovascular disease, pancreatic inflammation, and alcoholic fatty liver. (Ponnappa et al., 2000). Oxidative stress is one of the elements that are crucial in numerous pathways of alcohol-induced harm. The creation of ROS in our bodies is abnormally increased by our unhealthy eating habits and our way of life (smoking, drinking, obesity, and strenuous activity). When organisms experience oxidative stress brought on by free radical damage, antioxidants aid in coping. Antioxidant defenses come from two different sources: the diet, which includes fruits and vegetables, which are rich in vitamins C and E, carotenoids, ubiquinone, polyphenols, and lipoic acid. The other is endogenous and is made up of proteins, enzymes, or tiny molecules suchas glutathione, uric acid, superoxide dismutase, and glutathione peroxidase (ferritin, transferrin, etc.). Additionally, some elements that are significant cofactors include selenium, copper, and zinc. (Pincemail et al ., 2009). We were particularly interested in the plant Moringa oleifera and the edible fungus Pleurotus ostreatus because they contain significant antioxidant content. The plant Moringa oleifera Lam. (Moringaceae), also known as Nebeday in Senegal, is of Indian ancestry and is now common throughout Asia and Africa. The leaves are utilized in traditional African medicine and are commonly consumed as a legume. They are an excellent source of protein (19–35% dry matter) (Kane et al ., 2017; Makkar et al ., (1996); Abou-Elezz et al ., (2012) and are rich in metabolizable energy (2273–2978kcal/kg DM) (Makkar et al ., (1996); Olugbemi et al ., 2010). They A 11 Year 2022 Global Journal of Medical Research Volume XXII Issue II Version I ( D ) L © 2022 Global Journals are also rich in vitamins (A, B, C, and E), minerals (0.6–