Global Journal of Medical Research, L: Nutrition & Food Science, Volume 22 Issue 2

11.2% dry Matter). The South African ecotype of the plant has been observed to contain 19.3% crude protein (Moyo., 2011). Traditional Chinese medicine uses M. oleifera leaves to treat diabetes, headaches, fever, and malnourishment (Ndong et al ., 2007; Kerrarho., 1994). Preview studies have shown the health and nutritional interest of edible mushrooms (Zhang et al ., 2016; Alam et al ., 2008; Pornariya and Kanok, 2009). P. ostreatus has been demonstrated for its antitumor effects, antioxidant properties, antihyperlipidemic effects and antidiabetic effects, (Zhang et al ., 2016; Abrams et al., 2011; Alam et al ., 2008; Elmastas et al ., 2007; Jayakumar et al ., 2007; Jayakumar et al ., 2006). A daily intake of 15 g of dried oyster mushrooms would have an anti-hyperlipidemic effect on the subjects, it would also cover up to 50% of the recommended daily intakes of macronutrients and minerals, according to research on Pleurotus ostreatus nutritional value and antihyperlipidemic effects on HIV-positive individuals taking ARVs (Abrams et al ., 2011, Alam et al ., 2008; Manzi et al ., 2001; Alam et al ., 2009; Manzi et al ., 1999, Kane et al ., 2017). Given the rich nutrient, phytochemical, and organoleptic potential of M.oleifera and P.ostreatus , we designed this study to determine the antihyperlipidemic effect of Moringa oleifera leaves and Pleurotus ostreatus in Wistar rats stressed by a combination of Ethanol- paracetamol. In this paper, wewill code the dietary supplement by FMP16. II. M aterials and M ethods a) Plant Material and Preparation of the mixture of Leaves from M. oleifera and P. ostreatus The fresh leaves of M. oleifera were harvested at the botanical garden of the University Cheikh Anta Diop (UCAD) of Dakar, Senegal and identified at the botanical department (UCAD).The leaves were cleaned immediately after harvest, cut into small pieces, and dried in the shade for two weeks. The dried material was ground into a powder using a manual homogenizer. P. ostreatus were obtained by cultivation at the biotechnological laboratory of the University Cheikh Anta Diop of Dakar. The Moringa oleifera and Pleurotus ostreatus powders were combined in a 2:1 ratio to create the dietary supplement. The mixture was created following Kane et al, instructions (2017). The combination was dissolved in 0.01% starch paste before being fed to the rats. b) Animals and grouping Wistar rats strain to weigh 150 to 200 g were obtained from the Animal House of the National Institute of Youth and Sports in Yaounde. They were placed in plastic cages under standard laboratory conditions (temperature 20 to 30°C, relative air humidity 45 to 55%, and 12/12h light/dark cycle). The rats were fed with a basal diet and water ad libitum . The feed was a standard rat chow composed of carbohydrates (52%), protein (22%), fat (6.5%), water (12%), ash (6%), and fiber (4.5%). Every two days for 21 days, between 10:00 and 11:00 am, before the mixture administration of Moringa oleifera and Pleurotus ostreatus in proportion 2:1, made as reported by Kane et al ., the amount of food and water ingested by each group of rats as well as body weights were recorded (2017). The experiments were performed during the day (09am–03pm). c) Experimental design Thirty (30) adult male and female Wistar rats weighing 150 to 200 g were separated into five groups of six after two weeks of acclimatization: - Group 1 (TG): a stress-free control group that consumed only their regular diet of water, food, and vehicle (starch paste) once daily for 21 days, - Group 2 (TP): a control group that received paracetamol 12 hours after ethanol administration, was supplied in five sequential doses of 2 g. kg-1 using an orogastric tube to stress the group. For 21 days, they consumed the standard diet of water and food at their leisure in addition to the vehicle starch paste, - Group 3 (D1P): a group that received 500 mg/kg of FMP16 and was stressed by ethanol in five sequential doses of 2 g. kg -1 , administered through an orogastric tube; then received paracetamol 12 h after the last dose of ethanol. They received the standard diet (water and food ab-libitum) and the vehicle starch paste once a day for 21 days, - Group 4 (D2P): a group that received 1000 mg/kg of FMP16 and was stressed by ethanol in five sequential doses of 2 g. kg -1 , administered through an orogastric tube; then received paracetamol 12 h after the last amount of ethanol. They received the normal diet (water and food ab-libitum) and the vehicle starch paste once a day for 21 days, - Group 5 (D3P): a group that received 1500 mg/kg of FMP16 and was stressed by ethanol infive sequential doses of 2 g. kg -1 , administered through an orogastric tube; then received paracetamol 12 h after the last dose of ethanol. They received the standard diet (water and food ab-libitum) and the vehicle starch paste once a day for 21 days.The rats were given full access to food and water, and were on 12-hour light cycle each day (dark 12h-12h light). They were force-fed FMP16 using a gastroesophageal catheter and weighed every day. They fasted for the entire day before the animal sacrifice. On the 23rd day, the rats were given a night of rest before being slaughtered (while sedated with ether) by having their jugular veins cut. Organs such the liver, kidneys, brain, and testicles were collected along with blood. The liver, which was exclusively used in this 12 Year 2022 Global Journal of Medical Research Volume XXII Issue II Version I ( D ) L © 2022 Global Journals Antihyperlipidemic Property of a Dietary Supplement of Moringa Oleifera Leaves and Pleurotus Ostreatus in Wistar Rats Stressed by Combination of Ethanol-Paracetamol study, was wrung out, weighed, and stored at -20°C until