Global Journal of Medical Research, L: Nutrition & Food Science, Volume 22 Issue 2

processing day. It was then rinsed with ice-cold saline (0.9% NaCl) to eliminate any remaining blood. d) Determination of the biochemicals parameters in liver - Preparation of liver supernates Prior to biochemicals analysis, each liver sample was homogenized using a Poterproctor placed on ice and 10% homogenate was prepared using the KCL buffer solution (1.15%). The homogenates were centrifuged at 3000 rpm for 30 min at 4 ° C to collect the supernatant used for analysis. The supernatant of each sample was aliquoted in 1.5 ml Eppendorf tubes to estimatethe activity of antioxidant parameters (peroxidized lipids LPO, glutathione cellular GSH, catalase CAT). All liver parameters were expressed as activity per mg proteins. The proteins concentration in each fraction were determined by the method of (Gornall et al ., 1949) - Determination of biochemical parameters • Using the method of thiobarbituric acid-reacting substances, the mean malondialdéhyde (MDA) level (mol/mg protein), a measure of lipid peroxidation, was evaluated (Singh et al ., 2014). • The level of catalase activity was assayed by the method of Sinha (1972). • The level of Glutathionecellular activity was evaluated by the method of Ellman (1959). • Serum glutamyl oxaloacétate transaminase (SGOT) and serum glutamyl pyruvate transaminase (SGPT) activities were assayed by the method of Karmen et al ., (1955) and measured by standard essay kits SGM Italia Rome, Via Eschilo, 10139, (2012). • The albumin level was assayed by the method of Ferreria& Price (1974) and measured by standard essay kits Hospitex diagnostics, Via Arno, 4001010L, (2013). Creatinin level was assayed by the method of Bergmeyer (1987) and measured by standard essay kits Hospitex diagnostics, Via Arno, 4001621L (2014). • Testosterone level was assayed by the method of Tietz, (1986) and measured by Kit ELISA (DRG Diagnostics, Germany, EIA- 1559, (2009). • Total Cholesterol level was assayed by the method of Allain et al , (1974) and measured by standard essay kits Hospitex Diagnostics, Via Arno, 4001210L, (2011). • HDL Cholesterol level was assayed by the method of Grove (1979) and measured by standard essay kits SGM Italia, 10176, (2009). • Triglycerides level was assayed by the method of Babblok et al ., (1988) and measured by standard essay kits Fortress Diagnostics, United kingdom, BXC0271, (2013). e) Statistical Analysis IBM SPSS Statistics 20 software was used for statistical analysis and data processing. P-values less than 0.05 were regarded as significant in the statistical analysis, which was conducted using one-way analysis of variance (ANOVA) and Bonferroni's post-test for multiple comparisons. The results are presented as the mean and standard deviation (SD). III. R esults and D iscussion Results have shown that no significant difference was observed in final body weights (155– 173g) (Table1). Body weight gain ranged between 0.8 and 19 g for the four treatment groups. A decrease of 10% in the weight of D3N group was observed. These results corroborate those of Alam et al., (2011, 2009) who found that a diet enriched with Pleurotus ostreatus decreases the body weight of animals. However, Bobek et al., (1998) have shown that it does not affectthe weight as well as Schneider et al., (2011) who worked on humans with a daily dose of 30 g of dried oyster mushrooms, found that this does not affect anthropometric data. Bénissan et al., 2012, showed that the daily intake of 30 g of Moringa oleifera leaves improves nutritional recovery in children suffering from malnutrition. Hanaa et al., (2014), showed that a dose of 600 mg/kg of Moringa oleifera lowers the body mass index in obese subjects. Furthermore, the mixture of these species at a high dose of 1500 mg / kg, would explain the weight loss. This result was in contrast with those of Osman et al. (2012), who reported up to 14% changes in body weight of rats given M.oleifera extract for 21 days, attributing these changes to the rich nutrient quality of the extract. Results also have shown no significant difference in the amount of protein in the liver (figure1). Regarding lipid peroxidation (figure 2), results show no significant difference in the concentration of peroxidized lipids between the groups except between the unstressed control group (TG) and the 1500 mg/kg dose group where the concentration was 34% higher. These results are not in agreement with those of Mladenovic et al, (2013); Patere et al, (2011); Johnsen et al, (2007). The effect of FMP16 on oxidative stress enzymes such as catalase and cellular glutathione was also studied. Our results showed an increase in catalase activity of 87%, 85%, 90%, 82% respectively for the TG, D1P, D2P and D3P groups compared to the TP group (intoxicated and untreated). Also, catalase activity of the 1000 mg/kg dose (D2P) was 35% and 43% higher respectively compared to the 500 and 1500 mg/kg doses (figure 3 and 4). These results corroborate those of Lamou et al, (2015); Pornariya and Kanok, (2009); Elmastas et al, (2007); Mishra et al, (2011) and, they would be justified by the antioxidant capacity of both Moringa oleifera and Pleurotus ostreatus (Zhang et al., 13 Year 2022 Global Journal of Medical Research Volume XXII Issue II Version I ( D ) L © 2022 Global Journals Antihyperlipidemic Property of a Dietary Supplement of Moringa Oleifera Leaves and Pleurotus Ostreatus in Wistar Rats Stressed by Combination of Ethanol-Paracetamol 2016; Elmastas et al., 2007; Makkar et al., 1996; Sholapur and Patil, 2013). The liver damage caused by paracetamol, known as a hepatotoxic agent in case of overdose