Global Journal of Medical Research, L: Nutrition & Food Science, Volume 22 Issue 2

(Séide, 2008), is frequently used to assess the hepato- protective effects of medicinal plants (Lewerenz et al., 2003; Liu et al., 2011). The markers that are used to determine toxicity are usually transaminases (ALAT and ASAT), whose high concentration in the extracellular medium is synonymous with an alteration of the cells. In this study, our results showed that ALAT and ASAT activities were decreased in the FMP16 groups (Table 2). Thus, ALAT activity decreased by 29% in the 1000 mg/kg dose group compared to the intoxicated control. ASAT activity was higher in the intoxicated group (TP) by 28% and 26% compared to the 1000 and 1500 mg/kg doses. Compared to the results of the studies by Adedapo et al, (2009) and Alam et al, (2011), who instead found that a dose of 1600 mg/kg of Moringa oleifera leaves rather increased ALAT and ASAT activity; and on the other hand that a diet supplemented with 5% Pleurotus rather decreased transaminase activities, we could think that this explains the fact that FMP16 rather tends to regulate their activities due to the antagonistic effect that these two species have. Furthermore, results showed that the administration of FMP16 did not cause any significant difference in albumin and testosterone levels (Table 2). These results are similar to those of Alam et al, (2011) and Adedapo et al, (2009) who found that administration of Pleurotus and Moringa oleifera had no effect on albumin levels. Howewer, Prabsattro et al, (2015), Zade et al, (2013), Okolo et al, 2016 rather found in their studies that Moringa oleifera increases sexual performance and thus could be considered as a potential aphrodisiac. Regarding creatinine, FMP16 administration decreased creatinine levels in the treated groups (D1P, D2P, D3P) compared to the untreated and stressed group (TP) (Table 2). These results corroborate those of Sirag, (2009), Adedapo et al, (2009), Kane et al, 2022 who showed the protective effect of Pleurotus ostreatus and Moringa oleifera on kidney damage. Our results on lipid metabolism in rats revealed a significant decrease in total cholesterol in the 500, 1000 and 1500 mg/kg dose groups (figure 5). There was a 28%, 39%, 30% and 38% difference in TP, D1P, D2P and D3P compared to TG. In addition, a difference of 15% and 14% of D1P and D3P compared to TP. However, there was no significant difference in HDL cholesterol levels (Table 2). The results of the Triglycerides levels (Table 2) show a difference of 47% and 41% of the 500 and 1000 mg/kg dose compared to the TG control group. There was also a 28% decrease in Triglycerides levels at the 500 mg/kg dose compared to the dose 1000 mg/kg. In most of the studies on the effects of Pleurotus ostreatus and Moringa oleifera, they found a decrease in the concentration of LDL cholesterol which is more related to cardiovascular diseases (Bobek and Galbavy, 1999; Bobek et al., 1998; Hossain et al., 2003). These results are also in agreement with those of Alam et al, (2009), Schneider et al, (2011), Chumark et al, (2008), Kane et al, (2022). Our results (figure 6) and those of previous studies suggest that FMP16 would be an excellent cholesterol-lowering agent that could be recommended for the prevention and treatment of cardiovascular diseases. Table 1: Effect of the dietary supplement on rat weights GROUPES Starting Body weight (g) Final Body weight (g) P-value* TG 154 ± 3,34 173,67 ± 9,16 a 0, 02 TP 154,33±3,44 177,33±4,84 d 0,01 D1P 154±2,53 185,67±2,86 e 0,01 D2P 153,20±2,68 168±4,14 0,6 D3P 153±3,03 157±7,14 0, 13 *ANOVA test; TG: control group; TP: stressed group ethanol+paracetamol; D1P: stressed and treated group 500 mg/kg: D2P: stressed and treated group 1000 mg/kg; D3P: stressed and treated group 1500 mg/kg; a, d, e: mean statistically different with D3P à p <0,05 (test de Bonferoni) 14 Year 2022 Global Journal of Medical Research Volume XXII Issue II Version I ( D ) L © 2022 Global Journals Antihyperlipidemic Property of a Dietary Supplement of Moringa Oleifera Leaves and Pleurotus Ostreatus in Wistar Rats Stressed by Combination of Ethanol-Paracetamol Table 2: Effects of dietary supplement FMP16 on serum transaminases, albumin, testosterone, HDL-cholesterol and triglycerides activity GROUPS ALAT (U/I) ASAT (U/I) ALBUMINE (g/dl) TESTOS. (ng/dl) HDL-C (mg/dl) Triglyc. (mg/dl) TG 32,74±7,09 160,92±30,02 1,53±0,20 0,31±0,06 39,07±2,18 52,03±0,68 TP 48,48±3,32 196,35±33,40 1,61±0,37 0,47±0,12 38,88±1,88 78,14±12,69 D1P 43,16±8,80 209,16±25,74 1,80±0,10 0,53±0,08 36,30±2,89 71,02±18,92 D2P 34,31±4,55 141,86±11,19 1,60±0,20 0,44±0,04 35,08±2,53 98,18±7,64 D3P 43,66±2,13 145,86±22,20 1,75±0,18 0,43±0,03 34,17±5,47 87,83±21,97 The values are expressed as mean ± SD. TG: Control group rats with food and water ad libitum, TP: stressed rats without treatment, D1P: dose of 500 mg/kg, D2P : dose of 1000 mg/kg, D3P: dose of 1500 mg/kg.