Global Journal of Science Frontier Research, G: Bio-Tech & Genetics, Volume 22 Issue 2

N.B. After getting warfarin in the 3 rd step, you can use organic solvent to extract warfarin only then alkalize it using potassium base (potassium hydroxide); the OH group will be ionized into OK. a) Spectroscopic assay It is done by using nuclear magnetic resonance spectroscopy (NMR) to observe the local magnetic field around the atomic nuclei of the C13 atom. Warfarin potassium is placed in a magnetic field, and the signals are produced by exciting the nuclei sample of radio waves into NMR, which is detected with sensitive radio receivers. The intra-molecular area around the atom changes the frequency of resonance which gives access to the electronic structure of the molecule of warfarin potassium and its functional groups. Because the areas are like fingerprints as they are unique and very characteristic of each compound. III. R esults Warfarin potassium powder has an increased surface area with good bioavailability. As an oral anticoagulant, this drug has a prolonged duration of action because potassium has a molecular weight more than sodium, so the diameter of the potassium atom is bigger and electron negativity is lower, leading to a delayed onset of action; its chemical structure contains potassium atom, which may be safe to the hypertensive patients. Sodium atom can be substituted with potassium or lithium atom, which can help the patient relieve hypertension in addition to its essential role as an anticoagulant. Figure 13: The chemical shift of warfarin potassium showing estimation quality of each atom indicated by colors; blue is good, pink is medium, and red is rough a) Spectroscopic assay In a simple term, the paramagnetic NMR spectroscopy of warfarin potassium whose IUPAC name is potassium 2-oxo-3-(3-oxo-1-phenylbutyl)-2H- chromene-4-olate, was given a chemical shift range which was spanned up to thousands of parts per million. Figure 14: 1 H NMR spectroscopic assay of carbon atoms in warfarin potassium compound withchemical shift on the horizontal axis, each magnetically equivalent proton has a characteristic change and coupling to other protons appear as splitting of the peaks into multiplets between zero and 220 ppm Modulation of Warfarin Sodium into Warfarin Potassium for Patients with Hypertension © 2022 Global Journals 1 Year 2022 10 Global Journal of Science Frontier Research Volume XXII Issue ersion I VII ( G )