Global Journal of Science Frontier Research, G: Bio-Tech & Genetics, Volume 22 Issue 2

cumulating synthetic genome abs. Distances: 33548 Ratio synthetic genome abs. Distances / real genome abs. Distances = 14.38593482 TG/AC analysis Table 2: TG/AC Fibonacci and Lucas analysis for real NA1000 genome and synthetic Caulobacter 2.0. TG/CA Real genome NA1000 TG/CA Synthetic genome CAULOBACTER 2.0 Fibonacci Lucas Fibonacci Lucas 3 1607779 1614874 0.9956064684 3 1614874 1607779 1.00441292 3 296256 299993 0.9875430427 3 299993 296256 1.012614091 5 1349192 1358378 0.993237523 4 1011587 1022025 0.9897869426 5 244860 248873 0.9838753099 4 195156 199978 0.9758873476 8 915781 925976 0.9889899954 7 1174192 1180896 0.9943229548 8 169216 174299 0.9708374689 7 213326 216126 0.9870445944 13 642769 652548 0.9850141292 11 654997 665831 0.9837286038 13 120130 125243 959176 11 123819 128731 0.9618429127 21 384426 392873 0.9784994133 18 489376 497649 0.9833758332 21 73909 78870 0.9370990237 18 92670 96745 0.9578789602 34 209433 218299 0.9593859798 29 251102 259033 0.9693822795 34 42458 46179 0.9194222482 29 50145 53889 0.9305238546 55 91756 97158 0.9443998436 47 127067 133142 0.9543720239 55 20641 22427 0.9203638471 47 27305 29582 0.9230275167 89 31478 34303 0.917645687 76 45537 49636 0.9174188089 89 8188 9531 0.859091386 76 11334 12669 0.8946246744 Figure 2: Comparing TG/AC Fibonacci and Lucas distances in real and synthetic Caulobacter genomes ( regular- reverse distances weighted by the length of the genome, see detailed data in suplementary materials ) Part II: Mycoplasma Mycoides JCVI-syn1.0 (2010), JCVI- syn3.0 (2016) and JCVI-syn3A (2019) In 2010, a 1079-kb genome based on the genome of Mycoplasma mycoides (JCV-syn1.0) was chemically synthesized and supported cell growth when transplanted into cytoplasm. (Gibson, 2010) . In 2016, Hutchinson et al. design, build, and test cycle to reduce this Mycoplasma mycoides genome to 531 kb (473 genes). JCV-syn3.0 retains genes involved in key processes such as transcription and translation, but also contains 149 genes of unknown function. In the following section, we compare 6 (six) genomes : two reference real strain mycoplasma genomes including CAPRI strain, one transgenic building strain and the 3 strong JCV Labs ; synthetic genomes. 1 Year 2022 39 © 2022 Global Journals Global Journal of Science Frontier Research Volume XXII Issue ersion I VII ( G ) Epigenetics Theoretical Limits of Synthetic Genomes: The Cases of Artificials Caulobacter ( C. eth-2.0), Mycoplasma Mycoides (JCVI-Syn 1.0, JCVI-Syn 3.0 and JCVI_3A), E-coli and YEAST chr XII