Global Journal of Science Frontier Research, H: Environment & Earth Science, Volume 21 Issue 6

iii. Informed Consent Before the study began, the village heads together with the people of the communities were fully briefed on the objectives of the study. iv. Data Collection A structured questionnaire was used in collection of data required for the study. This contains questions on knowledge, attitude and practice which includes traditional in treatment of the infection. v. Urine Collection Dark (black), sterile, plastic universal containers (labeled) were given to the people both Children and Adult to collect urine samples. This was done between the hours of 10.00 am to 2.00 pm. The urine collected was taken to the laboratory for analysis. Each sample collected was visually examined for the presence of visible blood in urine. vi. Examination of Urine for Haematuria A reagent strip Medi-test Combi 9 was carefully dipped into the dark sterile bottle containing the urine for 5 seconds. The strip was drawn across the rim of the container to remove excess urine. After 30 to 60 seconds, the resulting change in color of the strip was compared with the manufacturers colour chart to estimate amount of blood in the urine. Each sample collected was visually examined for the presence of haematuria. vii. Examination for Schistosoma Haematobium Egg Ten (10) millitres of the urine sample was centrifuged at 2500 rpm for 10 minutes to separate the Schistosomahaematobium ova from the urine. The supernatant was discarded to leave sediment which was transferred to the centre of a clean grease-free glass slide. This was mounted on a light microscope and examined at x 10 and x 40 objectives to identify a Schistosomahaematobium ovum which is characterized with a terminal spine. viii. Statistical analysis of data The data was entered and analysed using SPSS version 20.0 for Windows. The questionnaire was analysed on Microsoft word excel using chi square. II. R esult A total of 152 people from the two communities were enrolled for the study (69 people from Ibaro and 83 people from Apojola) submitted their urine samples for analysis. The age range of the participants was from 1- 22 years above from both communities (Table 1). The prevalence in demographic information in respondents showing more female participated in the study than male in both communities with female 13(41.9%) and 31(63.3%) and male 18(58.1%) and 18(36.7%) were tested positive at Ibaro and Apojula communities respectively. The school aged children of 5-13 years had high number of population that participated in the study with 22(71.0%) and 20(40.8%) were tested positive. The major occupation of both communities are into fishing and farming, in the study high number of people are into fishing and 23(74.2%) and 39(79.6%) were tested positive in Ibaro and Apojula communities with no significant difference (p>0.05). Table 1: Demographic information of respondents and the prevalence of infection Traditional Approaches on Morbidity and Case Management of Urinary Schistosoma Infection in Rural Communities of Ogun State, Nigeria © 2021 Global Journals 1 Global Journal of Science Frontier Research Volume XXI Issue VI Year 2021 76 ( H ) Version I ________________________________________________________________________________ Location Ibaro Apojula Positive Negative Total Positive Negative Total _____________________________________________________________________________________ Sex M 18(58.1) 15(39.5) 33(47.8) 18(36.7) 23(67.6) 41(49.4) F 13(41.9) 23(60.5) 36(52.2) 31(63.3) 11(32.4) 42(50.6) P-value 0.151 P-value 0.007 Age 1-4 3(9.7) 8(21.1) 11(15.9) 10(20.4) 10(29.4) 20(24.1) 5-13 22(71.0) 22(57.9) 44(63.8) 20(40.8) 10(29.4) 30(36.1) 14-22 1(3.2) 4(10.5) 5(7.2) 8(16.3) 6(17.6) 14(16.9) <22 5(16.1) 4(10.5) 9(13.0) 11(22.4) 8(23.5) 19(22.9) P-value 0.319 P-value 0.699 Occupation Fishing 23(74.2) 26(68.4) 49(71.0) 39(79.6) 27(79.4) 66(79.5) Farming 2(6.5) 1(2.6) 3(4.3) 8(16.3) 5(14.7) 13(15.7) Others 6(19.4) 11(28.9) 17(24.6) 2(4.1) 2(5.9) 4(4.8) P-value 0.524 P-value 0.621 (n=152)